{"id":385482,"date":"2024-10-20T03:30:55","date_gmt":"2024-10-20T03:30:55","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-175472021\/"},"modified":"2024-10-26T06:22:51","modified_gmt":"2024-10-26T06:22:51","slug":"bs-en-175472021","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-175472021\/","title":{"rendered":"BS EN 17547:2021"},"content":{"rendered":"
This European Standard specifies a method for the determination of the content of the total vitamin A (retinol), vitamin E (alpha-tocopherol) and vitamin D (D2 ergocalciferol or D3 cholecalciferol) content in animal feed using solid phase extraction (SPE) clean-up and high performance liquid chromatography (HPLC). The limit of quantification is XXXX IU vitamin A\/kg (using UV-detection), XX IU vitamin A\/kg (using fluorescence detection), XX mg vitamin E\/kg (using UV-detection), XX mg vitamin E\/kg (using fluorescence detection), XX IU vitamin D\/kg (using UV-detection) and XX IU vitamin D\/kg (using fluorescence detection).<\/p>\n
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
8<\/td>\n | 4 Principle <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 5 Reagents and materials <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 6 Apparatus <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 7 Sampling <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 8 Sample preparation 9 Procedure <\/td>\n<\/tr>\n | ||||||
23<\/td>\n | 10 Expression of results <\/td>\n<\/tr>\n | ||||||
26<\/td>\n | 11 Observations <\/td>\n<\/tr>\n | ||||||
27<\/td>\n | 12 Precision <\/td>\n<\/tr>\n | ||||||
28<\/td>\n | 13 Test report <\/td>\n<\/tr>\n | ||||||
29<\/td>\n | Annex A (informative)Examples of combinations of weighing, aliquot and dilution to reach concentrations within the calibration curve <\/td>\n<\/tr>\n | ||||||
32<\/td>\n | Annex B (informative) Preparation of stock standard solution of vitamin E (\u03b1-tocopherol) from \u03b1-tocopherol acetate B.1 General B.2 Reagents B.3 Preparation of stock standard B.4 Standardization of the vitamin E (\u03b1-tocopherol) stock standard solution in cyclohexane <\/td>\n<\/tr>\n | ||||||
33<\/td>\n | B.5 Calibration solutions and plotting of calibration graph for vitamins A (retinol) and E (\u03b1-tocopherol) <\/td>\n<\/tr>\n | ||||||
34<\/td>\n | Annex C (informative)Results of the interlaboratory study <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Animal feeding stuffs: Methods of sampling and analysis. Determination of vitamin A, E and D content. Method using solid phase extraction (SPE) clean-up and highperformance liquid chromatography (HPLC)<\/b><\/p>\n |