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BS EN ISO 21150:2015

BS EN ISO 21150:2015

$142.49

ENISO 21150 Escherichia coli

Published By Publication Date Number of Pages
BSI 2015 26
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This International Standard gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621 ) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

The method described in this International Standard is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required.

NOTE

For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).

Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other International Standards ( ISO 18415 ) may be appropriate. Other methods (e.g. automated) can be substituted for the test presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.

PDF Catalog

PDF Pages PDF Title
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Diluents and culture media
5.1 General
11 5.2 Diluent for the bacterial suspension (tryptone sodium chloride solution)
5.2.1 General
5.2.2 Composition
5.2.3 Preparation
5.3 Culture media
5.3.1 General
5.3.2 Agar medium for the suitability test (see Clause 11) [soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA)]
12 5.3.3 Enrichment broth
5.3.4 Selective agar medium for isolation of Escherichia coli
13 5.3.5 Selective agar medium for confirmation of Escherichia coli
14 6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension in the enrichment broth
9.2.1 General
15 9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.2.4 Filterable products
9.3 Incubation of the inoculated enrichment broth
9.4 Detection and identification of Escherichia coli
9.4.1 Isolation
9.4.2 Identification of Escherichia coli
16 10 Expression of the results (detection of Escherichia coli)
11 Neutralization of the antimicrobial properties of the product
11.1 General
11.2 Preparation of inoculum
11.3 Suitability of the detection method
11.3.1 Procedure
17 11.3.2 Interpretation of suitability test results
12 Test report
18 Annex A (informative) Other enrichment broths
21 Annex B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
22 Bibliography

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BS EN ISO 21150:2015
$142.49