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BS EN 17194:2019:2020 Edition

$167.15

Animal feeding stuffs: Methods of sampling and analysis. Determination of Deoxynivalenol, Aflatoxin B1, Fumonisin B1 & B2, T-2 & HT-2 toxins, Zearalenone and Ochratoxin A in feed materials and compound feed by LC-MS/MS

Published By Publication Date Number of Pages
BSI 2020 36
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This document’s method of analysis is applicable for the determination of:

  • deoxynivalenol (DON) in the tested range of 100 μg/kg to 3 300 μg/kg,

  • aflatoxin B1 (AfB1) in the tested range of 2,5 μg/kg to 440 μg/kg,

  • fumonisin B1 (FB1) in the tested range of 690 μg/kg to 7 500 μg/kg,

  • fumonisin B2 (FB2) in the tested range of 200 μg/kg to 2 500 μg/kg,

  • T-2 toxin in the tested range of 7,5 μg/kg to 360 μg/kg,

  • HT-2 toxin in the tested range of 14 μg/kg to 1 800 μg/kg,

  • zearalenone (ZEN) in the tested range of 30 μg/kg to 600 μg/kg, and

  • ochratoxin A (OTA) in the tested range of 10 μg/kg to 230 μg/kg

in cereals and cereal-based compound feed by liquid-chromatography tandem mass spectrometry (LCMS/ MS). The actual working ranges could extend beyond the tested ranges.

PDF Catalog

PDF Pages PDF Title
2 undefined
6 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
7 5 Reagents
9 6 Apparatus
10 7 Procedure
7.1 Sample preparation
11 7.2 Extraction
7.2.1 Introduction
7.2.2 Dry samples
7.2.3 Slurry
7.3 Test solution
12 7.4 Spiking procedure
8 Measurements
8.1 General
8.2 LC conditions
8.3 MS conditions
13 8.4 Batch composition
8.5 Peak identification
8.6 Determination of mycotoxins in calibration and test solutions
8.7 Calibration
14 9 Determination of mass fraction
15 10 Precision
10.1 Interlaboratory study
10.2 Repeatability
16 10.3 Reproducibility
11 Test report
17 Annex A (informative)Precision data
27 Annex B (informative)Examples
B.1 General
B.2 Example 1
B.2.1 General
B.2.2 LC conditions
28 B.2.3 MS conditions
29 B.3 Example 2
B.3.1 General
B.3.2 LC conditions
30 B.3.3 MS conditions
32 Annex C (informative)Example chromatograms
BS EN 17194:2019
$167.15