BS EN 15788:2021 – TC:2022 Edition
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Tracked Changes. Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Enterococcus (E. faecium) spp. used as feed additive
Published By | Publication Date | Number of Pages |
BSI | 2022 | 56 |
This document specifies general rules for the enumeration of enterococci (E. faecium) in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain enterococci as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see A.2). The document is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) 767/2009) [4]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing 1011 CFU/kg; c) Compound feeds, meal or pellets which contain about 109 CFU/kg.
PDF Catalog
PDF Pages | PDF Title |
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34 | undefined |
40 | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle |
41 | 5 Diluents and culture media 5.1 Diluents |
42 | 5.2 Culture media |
44 | 6 Apparatus |
45 | 7 Sampling 8 Preparation of the test sample 9 Procedure 9.1 Preparation of poured agar plates for spread plate method |
46 | 9.2 Preparation of culture media for pour plate method 9.3 Preparation of the initial suspension and decimal dilutions |
47 | 9.4 Inoculation and incubation of the plates 9.4.1 General 9.4.2 Spread plate method |
48 | 9.4.3 Pour plate method 9.5 Enumeration of colonies 9.6 Confirmation |
49 | 10 Expression of results 11 Precision 11.1 General 11.2 Interlaboratory studies |
50 | 11.3 Repeatability 11.4 Reproducibility 12 Test report |
51 | Annex A (informative)Notes on the procedure A.1 General A.2 Critical copper concentration |
52 | Annex B (informative)Results of the interlaboratory studies B.1 Interlaboratory study based on bile aesculin azide agar B.2 Interlaboratory study based on Slanetz and Bartley agar |