API DR 148-1997

$39.00

Identification of Organic Toxicants in Treated Refinery Effluents

Published By	Publication Date	Number of Pages
API	1997	63

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Description

Toxicity characterization procedure with either larval fathead minnow (Pimephales promelas) or (Mysidopsis bahai) were performed with effluents from five refineries to identify non polar organic responsible for chronic toxicity. Test species selected for this study are also commonly used for determining compliance with effluent discharge toxicity limits. Any toxicity caused by more easily recognized substances, such as ammonia, was not of concern.

Characteristics if selected refinery effluents were initially examined to determine suitable effluents for identification of non polar organic toxicants. Non polar organic toxicants were operationally defined as those absorbed by C18 SPE Columns. Desirable effluent characteristics were:

1. Consistent presence of measurable chronic toxicity due to non polar organic compounds; and

2. A lack of toxicity from compounds other than non polar organics. Samples with these characteristics were preferred to minimize difficulties in tracking effluent toxicity through sample manipulations and to reduce the possibility if artifacts from multiple treatments required to address toxicants belonging to more than one class of compounds.

Historically, several common difficulties have been encountered during identification of non polar organic toxicants in refinery effluents. Past problems included:

1. Poor recovery of toxicity from C18 solid phase extraction (SPE) columns

2. Poor resolution of toxicity during superlative steps

3. Failure to recover toxicity following high performance liquid chromatography (HPLC) separation

4.inability to adequately simplify effluent fractions containing the non polar effluent fraction

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